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⚗️ Tris – Tris(hydroxymethyl)aminomethane Buffer
Tris (short for Tris(hydroxymethyl)aminomethane) is a widely used organic compound that serves as a buffering agent in biochemistry and molecular biology. Known for its effective pH-stabilizing properties, Tris is essential in applications like DNA/RNA extraction, protein purification, electrophoresis, and more.
🔬 Key Features:
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Chemical Formula: C₄H₁₁NO₃
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Molar Mass: 121.14 g/mol
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pKa (25°C): ~8.1, making it ideal for buffering between pH 7.0–9.0
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Solubility: Highly soluble in water
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Appearance: White crystalline powder or granules
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Applications:
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Buffering agent in biochemical research
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Common in Tris-HCl or Tris-EDTA (TE) buffers
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Ideal for electrophoresis, enzyme reactions, and cell lysis buffers
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🧪 Common Uses:
✔️ DNA & RNA preparation – maintains pH stability during extraction and purification
✔️ Electrophoresis – part of TAE or TBE buffers for gel electrophoresis
✔️ Protein work – stabilizes pH in solutions for protein assays or enzyme activity
✔️ Cell culture & lysis – used in buffers to maintain optimal cell environment
⚠️ Safety & Storage:
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Store in a cool, dry place in a tightly sealed container
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Avoid contact with eyes and prolonged skin exposure
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Handle with appropriate lab safety procedures
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