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  • ⚗️ Tris – Tris(hydroxymethyl)aminomethane Buffer

    Tris (short for Tris(hydroxymethyl)aminomethane) is a widely used organic compound that serves as a buffering agent in biochemistry and molecular biology. Known for its effective pH-stabilizing properties, Tris is essential in applications like DNA/RNA extraction, protein purification, electrophoresis, and more.


    🔬 Key Features:

    • Chemical Formula: C₄H₁₁NO₃

    • Molar Mass: 121.14 g/mol

    • pKa (25°C): ~8.1, making it ideal for buffering between pH 7.0–9.0

    • Solubility: Highly soluble in water

    • Appearance: White crystalline powder or granules

    • Applications:

      • Buffering agent in biochemical research

      • Common in Tris-HCl or Tris-EDTA (TE) buffers

      • Ideal for electrophoresis, enzyme reactions, and cell lysis buffers


    🧪 Common Uses:

    ✔️ DNA & RNA preparation – maintains pH stability during extraction and purification
    ✔️ Electrophoresis – part of TAE or TBE buffers for gel electrophoresis
    ✔️ Protein work – stabilizes pH in solutions for protein assays or enzyme activity
    ✔️ Cell culture & lysis – used in buffers to maintain optimal cell environment


    ⚠️ Safety & Storage:

    • Store in a cool, dry place in a tightly sealed container

    • Avoid contact with eyes and prolonged skin exposure

    • Handle with appropriate lab safety procedures

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